Part:BBa_K4687002:Design
The promoter PlacM
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The efficiency of homologous recombination of Corynebacterium glutamicum is very low, and non-homologous recombination will lead to uncertainty of gene editing. The current methods to improve the gene editing efficiency of Corynebacterium glutamicum are cumbersome and slow, but the recE and recT genes of λRed system or Rac prophage are recombined to greatly improve the gene editing efficiency in Escherichia coli. To this end, we need to improve the efficiency of gene editing for Corynebacterium glutamicum.The primary reason for using the recE/T system in conjunction with CRISPR-MAD7 is to enhance the efficiency and precision of genetic modifications.Through literature review, parallel experiments were conducted in related literature on the gene editing technology of Corynebacterium glutamicum. In combination with this project, through parallel experiment comparison, recE/T was finally selected to assist CRISPR-MAD7 in gene editing.
Source
recE: Bacterial,Archaeal and Plant Plastid Product: Rac prophage;exonuclease Ⅷ，5' to 3' specific dsDNA econuclease. recT: Bacterial,Archaeal and Plant Plastid Product: Rac prophage;recombination and repair protein.